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Fertilisation and embryo culture with in vitro fertilisation (IVF) were performed in F1 C3H/He-CBA hybrid mice to compare the calcium requirements of these aspects of reproduction between C3H/He and CBA mice. The egg donors were fed with either a control diet that was low in calcium or an experimental diet that was not only low in calcium but also zinc and magnesium. The experiments with both groups of mice showed that the fertilisation and the cleavage rates were similar to those of C3H/He mice, although the culture medium contained excess calcium for CBA mice. The calcium content of the fertilised oocytes at the pronuclear stage was only significantly lower in CBA mice, and the calcium content of the zygotes at the second pronuclear stage was only significantly lower in C3H/He mice, than in CBA mice. Neither the fertilisation rate nor the cleavage rate was affected by the diet. These results suggest that calcium supplementation for the culture medium may not be essential for the fertilisation and embryonic cleavage in our experimental conditions.Q:
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BY KELLY BILLITZ The Bulletin
Jun 5, 2013 at 11:16 AM
The East Hartford School District will commemorate the 25th anniversary of its Center School beginning at 9 a.m. Saturday, June 8, in the Commons Room at